Increased Expression of Chemerin in Squamous Esophageal Cancer Myofibroblasts and Role in Recruitment of Mesenchymal Stromal CellsReport as inadecuate




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Stromal cells such as myofibroblasts influence tumor progression. The mechanisms are unclear but may involve effects on both tumor cells and recruitment of bone marrow-derived mesenchymal stromal cells MSCs which then colonize tumors. Using iTRAQ and LC-MS-MS we identified the adipokine, chemerin, as overexpressed in esophageal squamous cancer associated myofibroblasts CAMs compared with adjacent tissue myofibroblasts ATMs. The chemerin receptor, ChemR23, is expressed by MSCs. Conditioned media CM from CAMs significantly increased MSC cell migration compared to ATM-CM; the action of CAM-CM was significantly reduced by chemerin-neutralising antibody, pretreatment of CAMs with chemerin siRNA, pretreatment of MSCs with ChemR23 siRNA, and by a ChemR23 receptor antagonist, CCX832. Stimulation of MSCs by chemerin increased phosphorylation of p42-44, p38 and JNK-II kinases and inhibitors of these kinases and PKC reversed chemerin-stimulated MSC migration. Chemerin stimulation of MSCs also induced expression and secretion of macrophage inhibitory factor MIF that tended to restrict migratory responses to low concentrations of chemerin but not higher concentrations. In a xenograft model consisting of OE21 esophageal cancer cells and CAMs, homing of MSCs administered i.v. was inhibited by CCX832. Thus, chemerin secreted from esophageal cancer myofibroblasts is a potential chemoattractant for MSCs and its inhibition may delay tumor progression.



Author: J. Dinesh Kumar, Chris Holmberg, Sandhir Kandola, Islay Steele, Peter Hegyi, Laszlo Tiszlavicz, Rosalind Jenkins, Robert J. Beyno

Source: http://plos.srce.hr/



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