Modulation of Human Colostrum Phagocyte Activity by the Glycine-Adsorbed Polyethylene Glycol MicrospheresReport as inadecuate

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Journal of ChemistryVolume 2013 2013, Article ID 845270, 8 pages

Research ArticleInstitute of Biological and Health Science, Federal University of Mato Grosso UFMT, Rodovia Br 070, Km 5 s-no, 78600-000 Barra do Garças, MT, Brazil

Received 18 January 2013; Revised 25 March 2013; Accepted 26 March 2013

Academic Editor: Hailong Xia

Copyright © 2013 Paulo Celso Leventi Guimarães et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Colostrum is a secretion that contains immunologically active components, including immunocompetent cells and glycine, which has anti-inflammatory, immunomodulatory, and cytoprotective effects. The aim of this study was to evaluate the adsorption of glycine onto polyethylene glycol PEG microspheres and to verify the immunomodulatory effect of this nanomaterial on human colostrum phagocytes. The PEG microspheres were evaluated by fluorescence microscopy. The effects of PEG microspheres with adsorbed glycine on viability, superoxide release, phagocytosis, microbicidal activity, and intracellular calcium release of mononuclear MN and polymorphonuclear PMN colostrum phagocytes were determined. Fluorescence microscopy analyses revealed that glycine was able to be adsorbed to the PEG microspheres. The PMN phagocytes exposed to glycine-PEG microspheres showed the highest superoxide levels. The phagocytes both MN and PMN displayed increased microbicidal activity and intracellular calcium release in the presence of PEG microspheres with adsorbed glycine. These data suggest that the adsorption of PEG microspheres with adsorbed glycine was able to stimulate the colostrum phagocytes. This material may represent a possible alternative therapy for future clinical applications on patients with gastrointestinal infections.

Author: Paulo Celso Leventi Guimarães, Adenilda Cristina Honorio-França, Cristiane de Castro Pernet Hara, Danny Laura Gomes Fagundes,



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