Expression of STAT5, COX-2 and PIAS3 in Correlation with NSCLC Histhopathological FeaturesReport as inadecuate

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Signal transducers and activators of transcription STATs, their inhibitors and cyclooxygenase-2 COX-2 participate in transformations of many various types of cancers. The aim of the present study was to evaluate the relationship between STAT5A-B, COX-2, and PIAS3 mRNA expression and tumor staging, metastasis status, and histopathological subtype in 71 patients with confirmed non-small cell lung cancer NSCLC diagnosis. Total RNA was isolated from NSCLC tissue samples and the expression of the studied genes was assessed using TaqMan probes in real-time PCR assay. The expression levels of STAT5A, STAT5B, and COX-2 genes were increased in 69%, 79%, and 71% NSCLC samples respectively, while PIAS3 expression was decreased in the majority 69% of the studied tissues. Statistically significant differences were observed between STAT5 isoforms P = 0.0008, with higher expression of STAT5B. We found statistically significant positive correlation between STAT5B and COX-2 rho = 0.045, and significant negative correlation between STAT5B and PIAS3 rho = −0.049. The negative correlation between STAT5B and PIAS3 rho = −0.43 was also observed in T2a+T2b tumor group. Additionally, STAT5B and COX-2 expression levels were significantly different between T1a+T1b and T2a+T2b tumors P = 0.002 and P = 0.041, respectively, with higher expression of both genes in T2 tumor stage. PIAS3 expression was significantly lower in NSCC subtype as compared with SCC subtype P = 0.017. Also, STAT5A and STAT5B immunoexpression was assessed, and the results indicated significantly higher protein levels in NSCLC patients as compared with controls P = 0.048 and P = 0.034, respectively. High STAT5B immunoexpression was positively correlated with STAT5B gene expression in tumors rho = 0.755. STAT5B protein level was also significantly higher in T2a+T2b tumors, reflecting high STAT5B gene expression in this group. There was no statistically significant association between mRNA and protein expression levels of the studied genes and patients- characteristics: age, gender, smoking. The obtained results highlight the importance of the genes STAT5B and COX-2 in lung cancer progression.

Author: Dorota Pastuszak-Lewandoska , Daria Domańska , Karolina H. Czarnecka, Jacek Kordiak, Monika Migdalska-Sęk, Ewa Nawrot, Justyna



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