Phylogenetic analysis of canine parvovirus isolates from Mathura, India.Report as inadecuate




Phylogenetic analysis of canine parvovirus isolates from Mathura, India. - Download this document for free, or read online. Document in PDF available to download.

Veterinary Archives, Vol.84 No.5 October 2014. -

Canine parvovirus CPV is an important pathogen causing acute hemorrhagic gastroenteritis in dogs and myocarditis in pups. The present study deals with an analysis of partial nucleotide sequences of the VP1-VP2 gene of CPV isolates from Mathura, India to establish their phylogenetic relationship with other CPV isolates. Out of 100 samples from dogs showing the clinical signs of gastroenteritis viz., vomiting, diarrhea and dysentery, 63 were found positive for CPV-2 by polymerase chain reaction PCR. Among the 63 positive samples, eight samples were processed further for nucleotide sequencing. Phylogenetic analysis revealed that the CPV variants were not only closely related among themselves but also showed minimum divergence from their ancestors, such as MEV, indicating very little divergence since their origin. From the study, it may be concluded that canine parvovirus-2 variants may represent a potential threat to canine populations. Thus more efforts is required to increase epidemiological monitoring and surveillance, along with the measures necessary

to control this disease in the canine population, and to assess the efficacy of the current vaccines.

canine parvovirus; dog; molecular characterization; PCR; phylogenetic analysis



Author: Deepti Singh - ; College of Biotechnology, Uttar Pradesh Pandit Deen Dayal Upadhayay Pashu Chikitsa Vigyan Vishvidhyalaya Evum Go

Source: http://hrcak.srce.hr/



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