Probing the In Vitro Cytotoxicity of the Veterinary Drug OxytetracyclineReport as inadecuate




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The study investigated the effect of oxytetracycline OTC on the anti-oxidative defense system, the structure hemolysis rate and morphology and function ATP enzyme activity of human red blood cells hRBCs to investigate the possible toxic mechanism of OTC to hRBCs. The experimental results indicate that OTC can cause a decline in the function of the antioxidant defense system of hRBCs, resulting in oxidative stress. OTC can bring about morphological changes to hRBCs, and further leads to hemolysis, when the concentration of OTC is over 8×10−5 M about 164 µg-ml. At a low OTC concentration, below 4×10−5 M 82 µg-ml, OTC can enhance the activity of ATP enzyme of hRBCs, known as hormesis. However, at a high concentration, above 4×10−5 M about 82 µg-ml, the ATP enzymatic activity was inhibited, affecting the function of hRBCs. The estalished mechanism of toxicity of OTC to hRBCs can facilitate a deeper understanding of the toxicity of OTC in vivo.



Author: Zhenxing Chi , Rutao Liu, Hong You, Shanshan Ma, Hao Cui, Qiang Zhang

Source: http://plos.srce.hr/



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