Preparation, Characterization, In Vitro Release and Degradation of Cathelicidin-BF-30-PLGA MicrospheresReport as inadecuate




Preparation, Characterization, In Vitro Release and Degradation of Cathelicidin-BF-30-PLGA Microspheres - Download this document for free, or read online. Document in PDF available to download.

Cathelicidin-BF-30 BF-30, a water-soluble peptide isolated from the snake venom of Bungarus fasciatus containing 30 amino acid residues, was incorporated in polyD,L-lactide-co-glycolide PLGA 75∶25 microspheres MS prepared by a water in oil in water W-O-W emulsification solvent extraction method. The aim of this work was to investigate the stability of BF-30 after encapsulation. D-trehalose was used as an excipient to stabilize the peptide. The MS obtained were mostly under 2 µm in size and the encapsulation efficiency was 88.50±1.29%. The secondary structure of the peptide released in vitro was determined to be nearly the same as the native peptide using Circular Dichroism CD. The ability of BF-30 to inhibit the growth of Escherichia coli was also maintained. The cellular relative growth and hemolysis rates were 92.16±3.55% and 3.52±0.45% respectively.



Author: Lili Li , Qifeng Wang , Hongli Li, Mingwei Yuan, Minglong Yuan

Source: http://plos.srce.hr/



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