γ-δ Cells in Fetal, Neonatal, and Adult Rat Lymphoid OrgansReport as inadecuate




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Developmental Immunology - Volume 4 1995, Issue 3, Pages 181-188



lnstitut für Virologie und Immunbiologie der Universität Würzburg, Versbacher Str. 7, Würzburg D-97078, Germany

Department of Cell Biology, Faculty of Biology, Complutense University, Madrid 28040, Spain

Received 22 November 1994; Accepted 13 January 1995

Copyright © 1995 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In the present study, we have analyzed the appearance and maturation of γ-δ T cells,recognized with a new mAb V65, in the central and peripheral lymphoid organs of fetal,neonatal, and adult Wistar rats. Cytofluorometrical analysis demonstrated the first V65

γ-δ T cells in the thymus of 16-17-day embryonic rats, although by immunohistology, theywere identified only in 19-day rat embryos in both the cortico-medullary border and thymicmedulla. Phenotypically, γ-δ thymocytes from fetal and neonatal thymus expressed CD3,CD2, and CD5, but only 60-80% were CD8

and approximately 40-50% expressed the αchain p55 of the IL-2R. In the periphery, the immunohistological study identified for thefirst time ,γ-δ T cells in the splenic white pulp and the gut of 21-day fetal rats, where theyoccurred within the epithelium as well as in the lamina propria. After birth, γ-δlymphocytes appeared in the skin, where they were present as dendritic epidermal T cellsin increasing numbers during postnatal life. Whereas these γ-δ T cells formed thepredominant T-cell population in the rat skin, γ-δ T cells in peripheral lymphoid organs,BALT, or the gut only represented a minor T-cell population. These results are discussed incomparison to γ-δ T cells of other vertebrate species.





Author: P. Kühnlein, A. Vicente, A. Varas, T. Hünig, and A. Zapata

Source: https://www.hindawi.com/



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