Development of Mouse Embryonic Stem ES Cells: IV Differentiation to Mature T and B Lymphocytes after Implantation of Embryoid Bodies Into Nude MiceReport as inadecuate




Development of Mouse Embryonic Stem ES Cells: IV Differentiation to Mature T and B Lymphocytes after Implantation of Embryoid Bodies Into Nude Mice - Download this document for free, or read online. Document in PDF available to download.

Developmental Immunology - Volume 4 1995, Issue 2, Pages 79-84



Basel Institute for Immunology, Grenzacherstrasser 487, Basel CH-4005, Switzerland

Centre d-Immunologie de Marseille-Luminy, Case 906, Marseille Cedex 9 F-13288, France

Received 10 May 1994; Accepted 31 October 1994

Copyright © 1995 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Mouse embryonic stem ES cells in culture can differentiate into late stages of manylineage-committed precursor cells. Under appropriate organ-culture conditions, ES celsdifferentiate into lymphoidlike cells at a stage equivalent to lymphoid cells found in fetalliver. These hematopoietic precursors are located in cup-shaped structures found in someembryoid bodies; we called such embryoid bodies -ES fetuses.- In this study, we havefollowed the maturation of hematopoietic cells after implantation of ES fetuses into nudemice for 3 weeks. ES-cell-derived lymphoid cells-pre-B cells, mature B cells, and mature Tcells were found in all lymphoid organs. Interestingly, there was also an increase of T cellsof host origin. Because native nude mouse lack thymus, these T cells might be educated bythymuslike epithelium generated from ES fetuses. Practical applications of this combined in vitro and in vivo system are discussed.





Author: Una Chen and Hoyan Mok

Source: https://www.hindawi.com/



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