Molecular Characterization of Escherichia coli Strains Isolated from Different Food SourcesReport as inadecuate




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Food Technology and Biotechnology, Vol.52 No.2 June 2014. -

Since food represents a possible source of pathogenic and antibiotic-resistant Escherichia coli strains, we analyzed 84 isolates from food samples identified in 2007 and 2008 at the National Institute of Public Health in Slovenia. Using polymerase chain reaction PCR, the isolates were classified into phylogenetic groups and subgroups following the Clermont method. Forty-two 50 % and thirty 35.7 % isolates were classified into commensal gut phylogenetic groups A and B1, respectively. Only ten 11.9 % and two 2.4 % isolates were assigned to the phylogenetic groups D and B2, which include mainly extraintestinal pathogenic E. coli strains. The strains were further analyzed for the presence of various virulence genes and plasmid-mediated quinolone resistance qnr genes. Virulence genes stx1, stx2, both stx1 and stx2, ehxA and eae associated with Shiga-toxin producing E. coli were detected in one 1.2 %, five 6 %, five 6 %, eight 9.5 % and three 3.7 % isolates, respectively. Seventy-four 88.1 % isolates carried the gene fimH, whereas virulence genes characteristic of extraintestinal pathogenic E. coli, hra, ompTAPEC and iha, were detected in nine 11 %, eight 9.5 % and six 7 % isolates, respectively. Genes kpsMTII, sfa, usp and vat were discovered in single isolates, whereas hlyA, bmaE, cnf, hbp and sat, as well as plasmid- mediated quinolone resistance genes qnr, were not detected in the analyzed strains. Our results show that various food items are indeed a source of intestinal and, albeit to a lesser extent, of extraintestinal pathogenic E. coli strains.

Escherichia coli; food; virulence genes; phylogenetic groups; plasmid-mediated quinolone resistance genes



Author: Marija Trkov - ; National Institute of Public Health of the Republic of Slovenia, Center for Laboratory Activities, Department of

Source: http://hrcak.srce.hr/



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