Ultrasensitive Electrochemical Immunoassay for Avian Influenza Subtype H5 Using NanocompositeReport as inadecuate




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We report a novel electrochemical immunosensor that can sensitively detect avian influenza virus H5 subtype AIV H5 captured by graphene oxide-H5-polychonal antibodies-bovine serum albumin GO-PAb-BSA nanocomposite. The graphene oxide GO carried H5-polychonal antibody PAb were used as signal amplification materials. Upon signal amplification, the immunosensor showed a 256-fold increase in detection sensitivity compared to the immunosensor without GO-PAb-BSA. We designed a PAb labeling GO strategy and signal amplification procedure that allow ultrasensitive and selective detection of AIV H5. The established method responded to 2−15 HA unit-50 µL H5, with a linear calibration range from 2−15 to 2−8 HA unit-50 µL. In summary, we demonstrated that the immunosenser has a high specificity and sensitivity for AIV H5, and the established assay could be potentially applied in the rapid detection of other pathogenic microorganisms.



Author: Zhixun Xie , Jiaoling Huang, Sisi Luo, Zhiqin Xie, Liji Xie, Jiabo Liu, Yaoshan Pang, Xianwen Deng, Qing Fan

Source: http://plos.srce.hr/



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