Comparison of Calcium and Barium Microcapsules as Scaffolds in the Development of Artificial Dermal PapillaeReport as inadecuate




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BioMed Research International - Volume 2016 2016, Article ID 9128535, 11 pages -

Research Article

Department of Histology and Embryology, Shantou University Medical College, Shantou, Guangdong 515000, China

Department of Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong 515000, China

Tissue Engineering Laboratory, The First Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong 515000, China

Department of Emergency, The Second Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong 515000, China

Received 27 October 2015; Revised 11 January 2016; Accepted 27 January 2016

Academic Editor: Richard Tucker

Copyright © 2016 Yang Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study aimed to develop and evaluate barium and calcium microcapsules as candidates for scaffolding in artificial dermal papilla. Dermal papilla cells DPCs were isolated and cultured by one-step collagenase treatment. The DPC-Ba and DPC-Ca microcapsules were prepared by using a specially designed, high-voltage, electric-field droplet generator. Selected microcapsules were assessed for long-term inductive properties with xenotransplantation into Sprague-Dawley rat ears. Both barium and calcium microcapsules maintained xenogenic dermal papilla cells in an immunoisolated environment and induced the formation of hair follicle structures. Calcium microcapsules showed better biocompatibility, permeability, and cell viability in comparison with barium microcapsules. Before 18 weeks, calcium microcapsules gathered together, with no substantial immune response. After 32 weeks, some microcapsules were near inflammatory cells and wrapped with fiber. A few large hair follicles were found. Control samples showed no marked changes at the implantation site. Barium microcapsules were superior to calcium microcapsules in structural and mechanical stability. The cells encapsulated in hydrogel barium microcapsules exhibited higher short-term viability. This study established a model to culture DPCs in 3D culture conditions. Barium microcapsules may be useful in short-term transplantation study. Calcium microcapsules may provide an effective scaffold for the development of artificial dermal papilla.





Author: Yang Liu, Changmin Lin, Yang Zeng, Haihong Li, Bozhi Cai, Keng Huang, Yanping Yuan, and Yu Li

Source: https://www.hindawi.com/



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