Resveratrol Metabolism in a Non-Human Primate, the Grey Mouse Lemur Microcebus murinus, Using Ultra-High-Performance Liquid Chromatography–Quadrupole Time of FlightReport as inadecuate




Resveratrol Metabolism in a Non-Human Primate, the Grey Mouse Lemur Microcebus murinus, Using Ultra-High-Performance Liquid Chromatography–Quadrupole Time of Flight - Download this document for free, or read online. Document in PDF available to download.

The grey mouse lemur Microcebus murinus is a non-human primate used to study the ageing process. Resveratrol is a polyphenol that may increase lifespan by delaying age-associated pathologies. However, no information about resveratrol absorption and metabolism is available for this primate. Resveratrol and its metabolites were qualitatively and quantitatively analyzed in male mouse-lemur plasma after 200 mg.kg−1 of oral resveratrol by ultra-high performance liquid chromatography UHPLC, coupled to a quadrupole-time-of-flight Q-TOF mass spectrometer used in full-scan mode. Data analyses showed, in MSE mode, an ion common to resveratrol and all its metabolites: m-z 227.072, and an ion common to dihydro-resveratrol metabolites: m-z 229.08. A semi-targeted study enabled us to identify six hydrophilic resveratrol metabolites one diglucurono-conjugated, two monoglucurono-conjugated, one monosulfo-conjugated and two both sulfo- and glucurono-conjugated derivatives and three hydrophilic metabolites of dihydro-resveratrol one monoglucurono-conjugated, one monosulfo-conjugated, and one both sulfo- and glucurono-conjugated derivatives. The presence of such metabolites has been already detected in the mouse, rat, pig, and humans. Free resveratrol was measurable for several hours in mouse-lemur plasma, and its two main metabolites were trans-resveratrol-3-O-glucuronide and trans-resveratrol-3-sulfate. Free dihydro-resveratrol was not measurable whatever the time of plasma collection, while its hydrophilic metabolites were present at 24 h after intake. These data will help us interpret the effect of resveratrol in mouse lemurs and provide further information on the inter-species characteristics of resveratrol metabolism.



Author: Marie-Claude Menet , Julia Marchal, Alexandre Dal-Pan, Méryam Taghi, Valérie Nivet-Antoine, Delphine Dargère, Olivier Laprévo

Source: http://plos.srce.hr/



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