MicroRNA Gene Polymorphisms and Environmental Factors Increase Patient Susceptibility to Hepatocellular CarcinomaReport as inadecuate




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Background

Micro RNAs miRNAs are small RNA fragments that naturally exist in the human body. Through various physiological mechanisms, miRNAs can generate different functions for regulating RNA protein levels and balancing abnormalities. Abnormal miRNA expression has been reported to be highly related to several diseases and cancers. Single-nucleotide polymorphisms SNPs in miRNAs have been reported to increase patient susceptibility and affect patient prognosis and survival. We adopted a case-control research design to verify the relationship between miRNAs and hepatocellular carcinoma.

Methodology-Principal Findings

A total of 525 subjects, including 377 controls and 188 hepatocellular carcinoma patients, were selected. Polymerase chain reaction-restriction fragment length polymorphism PCR-RFLP and real-time PCR were used to analyze miRNA146a rs2910164, miRNA149 rs2292832, miRNA196 rs11614913, and miRNA499 rs3746444 genetic polymorphisms between the control group and the case group. The results indicate that people who carry the rs3746444 CT or CC genotypes may have a significantly increased susceptibility to hepatocellular carcinoma adjusted odds ratio AOR = 2.84, 95% confidence interval CI = 1.88–4.30. In addition, when combined with environmental risk factors, such as smoking and alcohol consumption, interaction effects were observed between gene polymorphisms and environmental factors odds ratio OR = 4.69, 95% CI = 2.52–8.70; AOR = 3.38, 95% CI = 1.68–6.80.

Conclusions

These results suggest that a significant association exists between miRNA499 SNPs and hepatocellular carcinoma. Gene-environment interactions of miRNA499 polymorphisms, smoking, and alcohol consumption might alter hepatocellular carcinoma susceptibility.



Author: Yin-Hung Chu, Ming-Ju Hsieh, Hui-Ling Chiou, Yi-Sheng Liou, Chen-Chieh Yang, Shun-Fa Yang , Wu-Hsien Kuo

Source: http://plos.srce.hr/



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