Activated Peritoneal Cavity B-1a Cells Possess Regulatory B Cell PropertiesReport as inadecuate




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Previous studies have suggested that murine peritoneal cavity-derived B-1a cells possess similarities with described regulatory B cell subsets. The aim of the current study was to examine the potential immunoregulatory function of peritoneal cavity-derived B-1a cells. In vitro activation of peritoneal cavity-derived B- and B-1a cells shows that activation of these B cells with anti-CD40 and LPS induces these cells to secrete more IL-10, IL-6 and IgM as compared to splenic B cells. In a suppression assay, CD40-TLR4-activated peritoneal cavity B cells possess regulatory B cell functions as they inhibit the capacity of CD4+ T cells to produce both tumor necrosis factor-α and interferon-γ. Splenic B cells did not show this, whereas non-activated peritoneal cavity B cells augmented the capacity of CD4+ T cells to produce tumor necrosis factor-α, while the ability to produce interferon-γ was not altered. The current paper compares splenic B cells to peritoneal cavity B-1a cells in an in vitro activation- and an suppression-assay and concludes that peritoneal cavity B-1a cells possess properties that appear similar to splenic autoimmune-suppressive regulatory B cell subsets described in the literature.



Author: Bram Margry, Saskia C. W. Kersemakers, Aad Hoek, Ger J. A. Arkesteijn, Willemien H. Wieland, Willem van Eden, Femke Broere

Source: http://plos.srce.hr/



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