The Ability to Form Homodimers Is Essential for RDM1 to Function in RNA-Directed DNA MethylationReport as inadecuate




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RDM1 RNA-DIRECTED DNA METHYLATION1 is a small plant-specific protein required for RNA-directed DNA methylation RdDM. RDM1 interacts with RNA polymerase II Pol II, ARGONAUTE4 AGO4, and the de novo DNA methyltransferase DOMAINS REARRANGED METHYLTRANSFERASE2 DRM2 and binds to methylated single stranded DNA. As the only protein identified so far that interacts directly with DRM2, RDM1 plays a pivotal role in the RdDM mechanism by linking the de novo DNA methyltransferase activity to AGO4, which binds short interfering RNAs siRNAs that presumably base-pair with Pol II or Pol V scaffold transcripts synthesized at target loci. RDM1 also acts together with the chromatin remodeler DEFECTIVE IN RNA-DIRECTED DNA METHYLATION1 DRD1 and the structural-maintenance-of-chromosomes solo hinge protein DEFECTIVE IN MERISTEM SILENCING3 DMS3 to form the DDR complex, which facilitates synthesis of Pol V scaffold transcripts. The manner in which RDM1 acts in both the DDR complex and as a factor bridging DRM2 and AGO4 remains unclear. RDM1 contains no known protein domains but a prior structural analysis suggested distinct regions that create a hydrophobic pocket and promote homodimer formation, respectively. We have tested several mutated forms of RDM1 altered in the predicted pocket and dimerization regions for their ability to complement defects in RdDM and transcriptional gene silencing, support synthesis of Pol V transcripts, form homodimers, and interact with DMS3. Our results indicate that the ability to form homodimers is essential for RDM1 to function fully in the RdDM pathway and may be particularly important during the de novo methylation step.



Author: Taku Sasaki, Zdravko J. Lorković, Shih-Chieh Liang, Antonius J. M. Matzke, Marjori Matzke

Source: http://plos.srce.hr/



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