Streptomyces rimosus GDSL Lipase: Production, Heterologous Overexpression and Structure-Stability RelationshipReport as inadecuate




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Food Technology and Biotechnology, Vol.41 No.1 March 2003. -

Streptomyces rimosus lipase gene has been overexpressed in a heterologous host, S. lividans TK23. The maximal lipase activity was determined in the culture filtrates of the late stationary phase. Time course of lipase production was monitored by a modified plate assay. S. rimosus lipase gene has been located on the AseI B fragment approximately 2 Mb far from the left end of the S. rimosus linear chromosome. Out of eight examined streptomycetes, the presence of this rare type of bacterial lipase gene was detected in two belonging to the S. rimosus taxonomic cluster, and in one non-related species. Comparison of protein sequences of the Streptomyces lipolytic enzymes was performed. The result indicated the best structural stability of the putative S. coelicolor lipase-2.

lipase; Streptomyces; plate assay; thermostability



Author: Dušica Vujaklija - ; Ruđer Bošković Institute, Department of Molecular Genetics, Department of Organic Chemistry and Biochemi

Source: http://hrcak.srce.hr/



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