Novel Kinin B1 Receptor Splice Variant and 5′UTR Regulatory Elements Are Responsible for Cell Specific B1 Receptor ExpressionReport as inadecuate




Novel Kinin B1 Receptor Splice Variant and 5′UTR Regulatory Elements Are Responsible for Cell Specific B1 Receptor Expression - Download this document for free, or read online. Document in PDF available to download.

The kinin B1 receptor B1R is rapidly upregulated after tissue trauma or inflammation and is involved in cancer and inflammatory diseases such as asthma. However, the role of the: promoter; a postulated alternative promoter; and spliced variants in airway epithelial and other lung cells are poorly understood.We identified, in various lung cell lines and leucocytes, a novel, naturally occurring splice variant SV of human B1R gene with a shorter 5′untranslated region. This novel SV is ≈35% less stable than the wild-type WT transcript in lung adenocarcinoma cells H2126, but does not influence translation efficiency. Cell-specific differences in splice variant expression were observed post desArg10-kallidin stimulation with delayed upregulation of SV compared to WT suggesting potentially different regulatory responses to inflammation. Although an alternative promoter was not identified in our cell-lines, several cell-specific regulatory elements within the postulated alternative promoter region negative response element NRE −1020 to −766 bp in H2126; positive response element PRE −766 to −410 bp in 16HBE; −410 to +1 region acts as a PRE in H2126 and NRE in 16HBE cells were found.These findings reveal complex regulation of B1R receptor expression in pulmonary cells which may allow future therapeutic manipulation in chronic pulmonary inflammation and cancer.



Author: Faang Y. Cheah , Svetlana Baltic , Suzanna E. L. Temple, Kanti Bhoola, Philip J Thompson

Source: http://plos.srce.hr/



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