Morphogenetic Study on the Maturation of Osteoblastic Cell as Induced by Inorganic PolyphosphateReport as inadecuate




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Since inorganic polyphosphates polyP have an activity to induce bone differenciation in vitro and in vivo, we examined an effect of polyP on organelle by light microscopy and electron microscopy in Murine MC3T3-E1 osteoblastic cells. The MC3T3-E1 cells were ultrastructurally observed to possess morphological characteristics of osteoblasts. Cells cultured with polyP were strongly stained with an anti-collagen type I antibody but not in those cultured without polyP. Ultrastructural analysis of cells cultured with polyP revealed a well-developed Golgi apparatus, swollen and elongated rough endoplasmic reticulum, large mitochondria and many coated pits. Since MC3T3-E1 cells can be transformed from a resting phase to an active blastic cell phase after supplementation with polyP, it implies that polyP can be an effective material for bone regeneration.



Author: Kaori Tsutsumi , Nagahito Saito, Yumi Kawazoe, Hong-Kean Ooi, Toshikazu Shiba

Source: http://plos.srce.hr/



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