Relationship between Vitreous Levels of Matrix Metalloproteinases and Vascular Endothelial Growth Factor in Proliferative Diabetic RetinopathyReport as inadecuate




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To investigate which matrix metalloproteinases MMPs are more likely to be involved in the angiogenic process in proliferative diabetic retinopathy PDR, we measured the levels of MMPs in the vitreous fluid from patients with PDR and controls and correlated these levels with the levels of vascular endothelial growth factor VEGF. Vitreous samples from 32 PDR and 24 nondiabetic patients were studied by mosaic multiplex MMPs enzyme-linked immunosorbent assay ELISA, single ELISA, Western blot and zymography analysis. Epiretinal membranes from 11 patients with PDR were studied by immunohistochemistry. MMP-8 and MMP-13 were not detected. ELISA, Western blot and gelatin ymography assays revealed significant increases in the expression levels of MMP-1, MMP-7, MMP-9 and VEGF in vitreous samples from PDR patients compared to nondiabetic controls, whereas MMP-2 and MMP-3 were not upregulated in vitreous samples from PDR patients. Significant correlations existed between ELISA and zymography assays for the quantitation of MMP-2 r=0.407; p=0.039 and MMP-9 r=0.711; p<0.001. Significant correlations were observed between levels of VEGF and levels of MMP-1 r=0.845; P<0.001 and MMP-9 r=0.775; p<0.001, and between levels of MMP-1 and MMP-9 r=0.857; p<0.001. In epiretinal membranes, cytoplasmic immunoreactivity for MMP-9 was present in vascular endothelial cells and stromal monocytes-macrophages and neutrophils. Our findings suggest that among the MMPs measured, MMP-1 and MMP-9 may contribute to the angiogenic switch in PDR.



Author: Ahmed M. Abu El-Asrar , Ghulam Mohammad, Mohd. Imtiaz Nawaz, Mohammad Mairaj Siddiquei, Kathleen Van den Eynde, Ahmed Mousa, Gert

Source: http://plos.srce.hr/



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