SPRi-Based Strategy to Identify Specific Biomarkers in Systemic Lupus Erythematosus, Rheumatoid Arthritis and Autoimmune HepatitisReport as inadecuate




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Background

Heterogeneous nuclear ribonucleoprotein hnRNP A2-B1 is a target for antinuclear autoantibodies in systemic Lupus erythematosus SLE, rheumatoid arthritis RA, and autoimmune hepatitis AIH.

Aim

To monitor molecular interactions between peptides spanning the entire sequence of hnRNP A2-B1 and sera from patients and healthy controls.

Methods

Sera from 8 patients from each pathology and controls were passed across a surface plasmon resonance Imagery SPRi surface containing 39 overlapping peptides of 17 mers covering the human hnRNP B1. Interactions involving the immobilised peptides were followed in real time and dissociation rate constants koff for each interaction were calculated.

Results

Several significant interactions were observed: i high stability lower koff values between P55-70 and the AIH sera compared to controls p= 0.003; ii lower stability higher koff values between P118-133 and P262-277 and SLE sera, P145-160 and RA sera compared to controls p=0.006, p=0.002, p=0.007. The binding curves and koff values observed after the formation of complexes with anti-IgM and anti-IgG antibodies and after nuclease treatment of the serum indicate that i IgM isotypes are prevalent and ii nucleic acids participate in the interaction between anti-hnRNAP B1 and P55-70 and also between controls and the peptides studied.

Conclusions

These results indicate that P55-70 of hnRNP B1 is a potential biomarker for AIH in immunological tests and suggest the role of circulating nucleic acids, eg miRNA, present or absent according to the autoimmune disorders and involved in antigen-antibody stability.



Author: Elvire Beleoken, Hervé Leh, Armelle Arnoux , Béatrice Ducot , Claude Nogues , Eleonora De Martin, Catherine Johanet, Didier Sam

Source: http://plos.srce.hr/



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