Quantitative Analysis of BTF3, HINT1, NDRG1 and ODC1 Protein Over-Expression in Human Prostate Cancer TissueReport as inadecuate




Quantitative Analysis of BTF3, HINT1, NDRG1 and ODC1 Protein Over-Expression in Human Prostate Cancer Tissue - Download this document for free, or read online. Document in PDF available to download.

Prostate carcinoma is the most common cancer in men with few, quantifiable, biomarkers. Prostate cancer biomarker discovery has been hampered due to subjective analysis of protein expression in tissue sections. An unbiased, quantitative immunohistochemical approach provided here, for the diagnosis and stratification of prostate cancer could overcome this problem. Antibodies against four proteins BTF3, HINT1, NDRG1 and ODC1 were used in a prostate tissue array > 500 individual tissue cores from 82 patients, 41 case pairs matched with one patient in each pair had biochemical recurrence. Protein expression, quantified in an unbiased manner using an automated analysis protocol in ImageJ software, was increased in malignant vs non-malignant prostate by 2-2.5 fold, p<0.0001. Operating characteristics indicate sensitivity in the range of 0.68 to 0.74; combination of markers in a logistic regression model demonstrates further improvement in diagnostic power. Triple-labeled immunofluorescence BTF3, HINT1 and NDRG1 in tissue array showed a significant p<0.02 change in co-localization coefficients for BTF3 and NDRG1 co-expression in biochemical relapse vs non-relapse cancer epithelium. BTF3, HINT1, NDRG1 and ODC1 could be developed as epithelial specific biomarkers for tissue based diagnosis and stratification of prostate cancer.



Author: Andrew J. Symes, Marte Eilertsen, Michael Millar, Joseph Nariculam, Alex Freeman, Maria Notara, Mark R. Feneley, Hitenedra R. H.

Source: http://plos.srce.hr/



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