Stress Conditions Triggering Mucoid Morphotype Variation in Burkholderia Species and Effect on Virulence in Galleria mellonella and Biofilm Formation In VitroReport as inadecuate




Stress Conditions Triggering Mucoid Morphotype Variation in Burkholderia Species and Effect on Virulence in Galleria mellonella and Biofilm Formation In Vitro - Download this document for free, or read online. Document in PDF available to download.

Burkholderia cepacia complex Bcc bacteria are opportunistic pathogens causing chronic respiratory infections particularly among cystic fibrosis patients. During these chronic infections, mucoid-to-nonmucoid morphotype variation occurs, with the two morphotypes exhibiting different phenotypic properties. Here we show that in vitro, the mucoid clinical isolate Burkholderia multivorans D2095 gives rise to stable nonmucoid variants in response to prolonged stationary phase, presence of antibiotics, and osmotic and oxidative stresses. Furthermore, in vitro colony morphotype variation within other members of the Burkholderia genus occurred in Bcc and non-Bcc strains, irrespectively of their clinical or environmental origin. Survival to starvation and iron limitation was comparable for the mucoid parental isolate and the respective nonmucoid variant, while susceptibility to antibiotics and to oxidative stress was increased in the nonmucoid variants. Acute infection of Galleria mellonella larvae showed that, in general, the nonmucoid variants were less virulent than the respective parental mucoid isolate, suggesting a role for the exopolysaccharide in virulence. In addition, most of the tested nonmucoid variants produced more biofilm biomass than their respective mucoid parental isolate. As biofilms are often associated with increased persistence of pathogens in the CF lungs and are an indicative of different cell-to-cell interactions, it is possible that the nonmucoid variants are better adapted to persist in this host environment.



Author: InĂªs N. Silva, Andreia C. Tavares, Ana S. Ferreira, Leonilde M. Moreira

Source: http://plos.srce.hr/



DOWNLOAD PDF




Related documents