Optimized Method to Analyze Rose Plant Volatile Organic Compounds by HS-SPME-GC-FID-MSDReport as inadecuate




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A method involving Headspace solid-phase microextraction HS-SPME fiber combined with gas chromatography GC coupled with flame ionization detection FID and gas chromatography with mass spectrometry GC-MS was developed and optimized to investigate volatile organic compounds VOCs from different tissues flowers, leaves, stems, rhizosphere and whole plants of Floribunda and Hybrid Tea roses intact and cut. Three-phase fiber 50-30 μm divinylbenzene-carboxen-polydimethylsiloxane DVB-CAR-PDMS was used. Two types of chambers Tedlar bag and glass jar were evaluated for collection of VOCs and glass jar was selected. Absorbed compounds on the fiber were completely desorbed in the GC injector port at three desorption times 5, 10 and 15 min, and 5 min at 250?C was used. The maximum extraction efficiency for flowers tissues equilibrium absorption was achieved 2 h after fiber exposure in the headspace for intact and cut Floribunda and Hybrid Tea flowers. Under the optimized HS-SPME and GC-FID-MS conditions, 1h extraction time was chosen for intact and cut Floribunda and Hybrid Tea leaves and stems. The results demonstrated that 5 cm depth was selected for root and soil part rhizosphere for both rose cultivars, and 6 h and 12 h extraction time of VOCs from rhizosphere was achieved for Floribunda and Hybrid Tea, respectively. One hour was chosen for VOCs released from whole rose plants for both cultivars. In this study, the VOC profiles of two rose cultivars were characterized by the optimized HS-SPME-GC method. The different tissues of rose plants gave wide range of the VOCs; also the chromatograms of different cultivars were quite different and the specific VOC pattern of rose types depends on the species. Results from this study demonstrate the feasibility of this method for identifying VOCs from two rose cultivars and the potential use of this method for physiological studies on rose plants or on other floriculture plants.

KEYWORDS

Optimization, Rose tissues, VOCs, SPME-GC-FID-MS, Floribunda, Hybrid Tea

Cite this paper

Ibrahim, M. , Agarwal, M. , Hardy, G. and Ren, Y. 2017 Optimized Method to Analyze Rose Plant Volatile Organic Compounds by HS-SPME-GC-FID-MSD. Journal of Biosciences and Medicines, 5, 13-31. doi: 10.4236-jbm.2017.53003.





Author: Mohammed Ibrahim1,2, Manjree Agarwal1, Giles Hardy1, Yonglin Ren1*

Source: http://www.scirp.org/



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