Silencing of DLGAP5 by siRNA Significantly Inhibits the Proliferation and Invasion of Hepatocellular Carcinoma CellsReport as inadecuate




Silencing of DLGAP5 by siRNA Significantly Inhibits the Proliferation and Invasion of Hepatocellular Carcinoma Cells - Download this document for free, or read online. Document in PDF available to download.

Background

The dysregulation of oncogenes and tumor suppressor genes plays an important role in many cancers, including hepatocellular carcinoma HCC, which is one of the most common cancers in the world. In a previous microarray experiment, we found that DLGAP5 is overexpressed in HCCs. However, whether the up-regulation of DLGAP5 contributes to hepatocarcinogenesis remains unclear.

Methodology-Principal Findings

In this study, we showed that DLGAP5 was significantly up-regulated in 76.4% 168 of 220 of the analyzed HCC specimens when compared with adjacent liver tissue. DLGAP5 overexpression was evident in 25% 22 of 88 of the HCC specimens without AFP expression, suggesting that DLGAP5 may be a novel biomarker for HCC pathogenesis. The silencing of DLGAP5 gene expression by RNA interference significantly suppressed cell growth, migration and colony formation in vitro. The expression level of DLGAP5 was also found to be related to the methylation level of its promoter in the HCC specimens.

Conclusions-Significance

Taken together, these data suggest that the expression of DLGAP5 is regulated by methylation and that the up-regulation of DLGAP5 contributes to HCC tumorigenesis by promoting cell proliferation.



Author: Weijia Liao , Weilong Liu , Qing Yuan, Xing Liu, Ying Ou, Songqing He, Shengguang Yuan, Liling Qin, Qian Chen, Kate Nong, Minghui

Source: http://plos.srce.hr/



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