The Akt Pathway Inhibitor Degeulin Prevents Staphylococcal Enterotoxin B Induced Splenocyte Proliferation and InflammationReport as inadecuate




The Akt Pathway Inhibitor Degeulin Prevents Staphylococcal Enterotoxin B Induced Splenocyte Proliferation and Inflammation - Download this document for free, or read online. Document in PDF available to download.

Staphylococcal Enterotoxin B SEB is considered a potential biological weapon. It is toxic by both inhalation and ingestion. Effects of ingestion include fever, vomiting and diarrhoea, while inhalation may additionally result in chest pain, dyspnoea, pulmonary oedema and respiratory failure. Severe exposure may be fatal and treatment relies on symptomatic support. At a cellular level, SEB up-regulates T-cell proliferation leading to a pathological inflammatory response. Deguelin, a rotenoid isolated from the African plant Mundulea sericea Leguminosae, has been shown to reduce cellular proliferation by inhibiting the phosphoinositide 3-kinase-Akt PI3K-Akt signalling pathway. Using isolated murine splenocytes, we have demonstrated that treatment with deguelin reduces SEB inducing T cell proliferation by 60%. Deguelin treatment also decreased IL-2 and CCL2 secretion by splenocytes exposed to SEB. We demonstrate that targeting cellular proliferation can significantly reduce inflammation after SEB exposure and suggest that anti-proliferatives may have a role as potential generic medical counter measures if superantigens are used as biological weapons.

KEYWORDS

Staphylococcal Enterotoxin B, Deguelin, Therapy, Inflammation, Biological Weapon

Cite this paper

Whitfield, S. , Risdall, J. , Griffiths, G. , Williamson, E. and Carter, A. 2017 The Akt Pathway Inhibitor Degeulin Prevents Staphylococcal Enterotoxin B Induced Splenocyte Proliferation and Inflammation. Advances in Bioscience and Biotechnology, 8, 1-12. doi: 10.4236-abb.2017.81001.





Author: Sarah Joanne Christine Whitfield1*, Jane Elizabeth Risdall1,2, Gareth Griffiths1, Ethel Diane Williamson1, Alun James Carter1,3

Source: http://www.scirp.org/



DOWNLOAD PDF




Related documents