Tailor-Made Electrospun Culture Scaffolds Control Human Neural Progenitor Cell Behavior—Studies on Cellular Migration and Phenotypic DifferentiationReport as inadecuate




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In neuroscience research, cell culture systems areessential experimental platforms. It is of great interest to explore in vivo-like culture substrates. Weexplored how basic properties of neural cells, nuclei polarization, phenotypicdifferentiation and distribution-migration, were affected by the culture atpoly-L-lactic acid PLLA fibrous scaffolds, using a multipotentmitogen-expanded human neural progenitor cell HNPC line. HNPCs were seeded,at four different surfaces: two different electrospun PLLA d = 1.2 - 1.3 μm substratesparallel or random aligned fibers, and planar PLL- and PLLA surfaces. Nucleianalysis demonstrated a non-directed cellular migration at planar surfaces andrandom fibers, different from cultures at aligned fibers where HNPCs wereoriented parallel with the fibers. At aligned fibers, HNPCs displayed the samecapacity for phenotypic differentiation as after culture on the planarsurfaces. However, at random fibers, HNPCs showed a significant lower level ofphenotypic differentiation compared with cultures at the planar surfaces. Aclear trend towards greater neuronal formation at aligned fibers, compared tocultures at random fibers, was noted. We demonstrated that the topography of in vivo-resembling PLLA scaffoldssignificantly influences HNPC behavior, proven by different migration behavior,phenotypic differentiation potential and nuclei polarization. This knowledge isuseful in future exploration of in vivo-resemblingneural cell system using electrospun scaffolds.

KEYWORDS

Human Stem Cells, PLLA, Electrospinning, Differentiation, Migration

Cite this paper

Englund-Johansson, U. , Netanyah, E. and Johansson, F. 2017 Tailor-Made Electrospun Culture Scaffolds Control Human Neural Progenitor Cell Behavior—Studies on Cellular Migration and Phenotypic Differentiation. Journal of Biomaterials and Nanobiotechnology, 8, 1-21. doi: 10.4236-jbnb.2017.81001.





Author: Ulrica Englund-Johansson1*, Eitan Netanyah1,2, Fredrik Johansson2

Source: http://www.scirp.org/



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