Isolation and Identification of Alkali-Resistant 1,3-Propanediol Producing StrainReport as inadecuate




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1,3-Propanediol is a promising renewable resource produced by microbial production. It is mainly used in many synthetic reactions, particularly applied to the polymer synthesis and cosmetics industry. We described here the isolation of strain ZH-1, which has the ability of high production with 1,3-propanediol, from Fenhe River in China. It was classified as a member of K. pneumoniae after the study of phenotypic, physio-logical, biochemical and phylogenetic 16S rDNA. The initial glycerol concentration, fermentation time and pH value of strain ZH-1 were determined to be 50 g·L-1, 36 h and 8.0. Under these conditions, the practical yield of 1,3-PD was 18.53 g·L-1 and a molar yield mol1,3-PD molGlycerol-1 of 1,3-propanediol to glycerol of 0.497. In addition, we found that for the strain ZH-1, the optimum grown pH was 9.0, so we can deter-mine that it is a new member of alkali-resistant strains.

KEYWORDS

1, 3-Propanediol, K. pneumoniae, Identification, Alkali-Resistant

Cite this paper

Zhao, Z. , Wen, C. , Rong, G. , Liu, R. , Xu, J. and Hu, Q. 2016 Isolation and Identification of Alkali-Resistant 1,3-Propanediol Producing Strain. Advances in Microbiology, 6, 917-926. doi: 10.4236-aim.2016.612086.





Author: Zhifang Zhao, Caifang Wen, Guang Rong, Ruiqing Liu, Jianguo Xu, Qingping Hu*

Source: http://www.scirp.org/



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