Decreased Expression of the FOXO3a Gene Is Associated with Poor Prognosis in Primary Gastric Adenocarcinoma PatientsReport as inadecuate




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Background

FOXO3a, a member of the forkhead class ‘O’ FOXO transcription factor family, controls a wide spectrum of biological processes, such as DNA damage repair, apoptosis, and cell cycle regulation. FOXO3a has been shown to be a tumor suppressor in various cancers. This study investigated the expression of FOXO3a in primary gastric adenocarcinomas and its prognostic value for primary gastric adenocarcinoma patients.

Methods

Real-time quantitative RT-PCR qRT-PCR, western blotting, and immunohistochemical staining were used to detect FOXO3a expression in primary gastric cancerous surgical specimens and adjacent non-tumorous tissues.

Results

Our data showed that the expression of FOXO3a mRNA p = 0.03 and protein p = 0.019 was lower in cancerous tissues compared with their adjacent non-tumorous tissues. In addition, the chi-square test revealed that low FOXO3a expression was significantly correlated with larger tumor size p = 0.007, poor histopathological classification p = 0.029, depth of invasion p = 0.049, local lymph node metastasis p = 0.013, distant metastasis p = 0.013 and AJCC staging p<0.001. Kaplan-Meier survival analysis demonstrated that low expression of FOXO3a was significantly correlated with a poor prognosis for gastric cancer patients p<0.001. The multivariate analysis showed that FOXO3a expression was an independent prognostic factor of the overall survival rate of patients with primary gastric adenocarcinoma.

Conclusion

Our study suggested that decreased FOXO3a expression may play an important role in the progression of gastric cancer. FOXO3a could be a valuable prognostic marker as well as a potential molecular therapy target for gastric cancer patients.



Author: Xiao-bo Yang , Jing-jing Zhao , Chun-yu Huang , Qi-jing Wang, Ke Pan, Dan-dan Wang, Qiu-zhong Pan, Shan-shan Jiang, Lin Lv, Xiang

Source: http://plos.srce.hr/



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