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NaturalOrganic Matter NOM is a mixture of aromatic and aliphatic organic compoundsof natural origin in any type of aquatic system. Human activities impact theconstituents of NOM, from its production to its fate, particularly in the treatmentof domestic waste waters. In this work, the impact of microorganisms isolatedfrom a Waste Water Treatment Plant WWTP was investigated to determine thefate of NOM fractions in raw sewage, using fluorescence spectroscopy. Wastewatersamples were taken at three different times from a WWTP, and incubated for 4days under two treatments: 1 -raw sewage-, and 2 -spiked-, i.e., the same raw sewage, spiked withbacteria previously isolated from the WWTP. The incubated waters were analyzedby fluorescence spectroscopy, digitally resolved into NOM components: humic-and fulvic-like, and two types of protein-like, i.e., tryptophan- and tyrosine-like, using a Parallel FactorAnalysis routine PARAFAC. The results demonstrate that the -spiked- samplesshowed the largest changes with incubation time. The signals of the tryptophan-and tyrosine-like components decreased, suggesting a net microbial digestion ofproteinaceous material. In contrast, the fulvic-like signals, and to someextent, the humic-like signals increased, suggesting the production of theassociated molecular materials during the incubation period. This studyprovides direct evidence of human impact on the make-up of NOM: the culturesof microbes found at a WWTP consume the proteinaceous material, whereashumic-like and fulvic-like materials are produced.

KEYWORDS

Natural Organic Matter, Wastewater, Microbial Utilization, Fulvic-Like, Protein-Like, Incubation, Fluorescence, PARAFAC

Cite this paper

Riopel, R. , Siemann, S. and Caron, F. 2016 Microbial Changes in the Fluorescence Character of Natural Organic Matter from a Wastewater Source. Journal of Water Resource and Protection, 8, 873-883. doi: 10.4236-jwarp.2016.89072.





Author: Rémi Riopel1,2, Stefan Siemann1, François Caron1,3*

Source: http://www.scirp.org/



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