Self-consistent theory of reversible ligand binding to a spherical cell - Quantitative Biology > Subcellular ProcessesReport as inadecuate




Self-consistent theory of reversible ligand binding to a spherical cell - Quantitative Biology > Subcellular Processes - Download this document for free, or read online. Document in PDF available to download.

Abstract: In this article, we study the kinetics of reversible ligand binding toreceptors on a spherical cell surface using a self-consistent stochastictheory. Binding, dissociation, diffusion and rebinding of ligands areincorporated into the theory in a systematic manner. We derive explicitly thetime evolution of the ligand-bound receptor fraction pt in various regimes .Contrary to the commonly accepted view, we find that the well-knownBerg-Purcell scaling for the association rate is modified as a function oftime. Specifically, the effective on-rate changes non-monotonically as afunction of time and equals the intrinsic rate at very early as well as latetimes, while being approximately equal to the Berg-Purcell value atintermediate times. The effective dissociation rate, as it appears in thebinding curve or measured in a dissociation experiment, is strongly modified byrebinding events and assumes the Berg-Purcell value except at very late times,where the decay is algebraic and not exponential. In equilibrium, the ligandconcentration everywhere in the solution is the same and equals its spatialmean, thus ensuring that there is no depletion in the vicinity of the cell.Implications of our results for binding experiments and numerical simulationsof ligand-receptor systems are also discussed.



Author: Shivam Ghosh St.Stephens College, Delhi, Manoj Gopalakrishnan HRI, Allahabad, Kimberly Forsten-Williams Virginia Tech

Source: https://arxiv.org/



DOWNLOAD PDF




Related documents