Construction and Analysis of the Immune Effects of a Streptococcus agalactiae Surface Protein ScpB Vaccine Encapsulated with Polylactic-Co-Glycolic Acid PLGAReport as inadecuate




Construction and Analysis of the Immune Effects of a Streptococcus agalactiae Surface Protein ScpB Vaccine Encapsulated with Polylactic-Co-Glycolic Acid PLGA - Download this document for free, or read online. Document in PDF available to download.

In order to find an effective immune preparation to controltilapia streptococcus disease, the Streptococcus agalactiae surface protein serine proteaseC5a peptidase ScpB was cloned and the recombinant protein was encapsulated inpoly lactide-co-glycolic acid PLGA microspheres, which were comprised of biodegradablematerials. The ScpB-PLGA vaccine was then administered to the tilapia intraperitoneallyat different concentrations, with PBS used as a control, and the relative percentsurvival RPS of each group was calculated. Serum lysozyme and superoxide dismutaseSOD activity levels and antibody levels OD450nm were tested weeklyfor the duration of the experiment. The results showed that the ScpB loading ratein the PLGA microspheres was 2.55% and the encapsulation efficiency reached 48.76%.The RPS ranged from 66.80% to 87.66%, with the highest RPS noted in group P1 1μg-g. The serum lysozyme, SOD and antibody IgM levels were significantly higherin the vaccinated fish relative to the control groups P < 0.01. These results showed that PLGA could serve as an effectiveadjuvant for a ScpB vaccine and could provide relatively sustained immune protection.

KEYWORDS

Tilapia, Streptococcus agalactiae, C5a Peptidase, Immunogenicity

Cite this paper

Ke, X. , Li, Q. , Li, X. , Liu, Z. , Lu, M. and Yang, H. 2016 Construction and Analysis of the Immune Effects of a Streptococcus agalactiae Surface Protein ScpB Vaccine Encapsulated with Polylactic-Co-Glycolic Acid PLGA. Open Access Library Journal, 3, 1-11. doi: 10.4236-oalib.1102886.





Author: Xiaoli Ke1,2, Qingyong Li2, Xiaotian Li2, Zhigang Liu2, Maixin Lu2*, Hong Yang1*

Source: http://www.scirp.org/



DOWNLOAD PDF




Related documents