Metabolic Effects of Endogenous and Exogenous Heterotropic Hemoglobin Modulators on Anion Transport: The Case of Pig ErythrocytesReport as inadecuate




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This comparativestudy, focused on the anion kinetic of band 3 protein of pig erythrocytes, investigates in vitro the metabolic functionalityof the red blood cells of this mammal which show particular characteristics ofthe cell membrane and significant stoichiometric variations of severalcytosolic components. Pig red blood cells compared to human ones, arecharacterized by a higher anion flux strongly modulated by the conformationaltransition state T-R of hemoglobin; these findings are probably connected tothe intracellular concentration of 2,3-disphosphoglycerate and to chlorideactivity. The experimental use, alone or in combination, of some exogenousheterotropic modulators of hemoglobin as orthovanadate, gemfibrozil andresveratrol, shows characteristic modulations of the anion flux which arerelated to the cytosolic metabolites physiologically present in pig erythrocytes,to the metabolism and oxidative stress. Our findings highlight new featuresabout the pig red blood cells functionality and contribute to extending thecurrent understanding of their life cycle, providing opportunities for newinvestigations in the field of comparative physiology.

KEYWORDS

Band 3 Protein, 2, 3-Diphosphoglycerate, Hemoglobin, Metabolism, Red Blood Cell

Cite this paper

Tellone, E. , Russo, A. , Giardina, B. , Galtieri, A. and Ficarra, S. 2015 Metabolic Effects of Endogenous and Exogenous Heterotropic Hemoglobin Modulators on Anion Transport: The Case of Pig Erythrocytes. Open Access Library Journal, 2, 1-11. doi: 10.4236-oalib.1101994.





Author: Ester Tellone1*, Annamaria Russo1, Bruno Giardina2,3, Antonio Galtieri1, Silvana Ficarra1

Source: http://www.scirp.org/



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