Infectious Spondylitis-Associated Staphylococcus aureus with Virulence Gene pvl or tst Causes More Necrosis than Apoptosis in Human Alveolar Basal Epithelial Cell Line A549Report as inadecuate




Infectious Spondylitis-Associated Staphylococcus aureus with Virulence Gene pvl or tst Causes More Necrosis than Apoptosis in Human Alveolar Basal Epithelial Cell Line A549 - Download this document for free, or read online. Document in PDF available to download.

Methicillin-sensitive and resistant Staphylococcus aureus MSSA and MRSA, respectively can cause non-tuberculosis infectious spondylitis. In43 cases of bacterial infectious spondylitis, Mycobacterium tuberculosis and S.aureus were the two major causative pathogens. MRSA caused more anterioroperations and thoracic infections, while MSSA caused more posterior infectionsand lumbar infections. Differences between six S. aureus isolates from infectious spondylitis were characterized.MLST and staphylococcal cassette chromosome mec SCCmec analysis identified MSSA ST959 and ST30 isolates, MRSAST239-SCCmec IIIA isolates 2 and 3, ST59-SCCmec IIIA-like isolate 6, and ST30-SCCmec IV isolate 5. Whileall of the isolates were resistant to penicillin and ampicillin, theMRSA isolates were more resistant than the MSSA isolates. Carbapenem-resistantMRSA ST239-SCCmec IIIA and ST59-SCCmec IIIA-like isolates of the agr1 type were also resistant to clindamycin and erythromycin. Leukocidin genes pvl or lukED and hemolysingenes hla, hld and hlg were present inall of the isolates. All six isolates caused more necrosis than apoptosis in thehuman alveolar basal epithelialcell line A549; however,ST59-SCCmec IIIA-like isolate 6,ST30- SCCmec IV isolate 5 with pvl genes, and MSSA ST30 isolates with tst caused greater than 40% cell deathafter the 4-h incubation. Regardless of the MRSA isolate and its SCCmec type or the MSSA isolate, the infectious spondylitis-associated S. aureus isolates differed genetically, and the pvl and tst genes may be important genesfor cell necrosis.

KEYWORDS

Infectious Spondylitis, Staphylococcus aureus, Virulence Factor, MLST, Necrosis, Apoptosis

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Huang, T. , Lee, C. , Wu, M. , Li, Y. , Yang, T. , Cheng, C. , Lee, C. , Lu, C. and Chu, C. 2016 Infectious Spondylitis-Associated Staphylococcus aureus with Virulence Gene pvl or tst Causes More Necrosis than Apoptosis in Human Alveolar Basal Epithelial Cell Line A549. Advances in Microbiology, 6, 479-488. doi: 10.4236-aim.2016.67047.





Author: Tsung-Jen Huang1,2,3,4, Chi-Han Lee5, Meng-Huang Wu1,3,4, Yen-Yao Li1,2, Tsung Han Yang6, Chin-Chang Cheng1,2, Ching-Yu Lee1, Chi

Source: http://www.scirp.org/



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