Effect of Hyperglycemia on Erythrocyte Carbonic Anhydrase and Lactic Acid in Type II Diabetic SubjectsReport as inadecuate




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Background: Carbonic anhydrase CA is a ubiquitous enzyme catalyzing thereversible hydration of CO2 to  and H+. CA plays a crucial role inCO2 transport, acid-base balance, and in linking local acidosis to O2 unloading from hemoglobin and also facilitates lactate shuttling across themonocarboxylate transporters MCT. The study aimed to investigate theinfluence of hyperglycemia on erythrocyte carbonic anhydrase activity andlactic acid in type II diabetic patients. Method: Red blood cell carbonicanhydrase activity was determined in washed lysed-hemolysate by the action ofthe enzyme on the substrate p-nitrophenyl acetate. The absorbance of releasedp-nitrophenol was recorded at 345 nm. Glycated Hemoglobin was determined by ionexchange method Spectrum Diagnostic Kit. Blood glucose, lactate, cholesteroland triglyceride were determined using Accutrend GCT meter Roche, Germanywith cobias® test strips. Results: The present study showed thathyperglycemia significantly p < 0.05 increased both erythrocyte carbonicanhydrase activity and blood lactate level in type II diabetic patients.Conclusion: We may conclude that hyperglycemia may be responsible for theincreased activity of carbonic anhydrase activity and blood lactateconcentration.

KEYWORDS

Carbonic Anhydrase, Lactic Acid, Glycated Hemoglobin, Type II Diabetes

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Ibrahim, S. , Amodu, A. , Ene-Ojo, A. , Ismaila, U. and Fakhruddeen, M. 2016 Effect of Hyperglycemia on Erythrocyte Carbonic Anhydrase and Lactic Acid in Type II Diabetic Subjects. Journal of Diabetes Mellitus, 6, 158-165. doi: 10.4236-jdm.2016.62017.





Author: Salihu Ismail Ibrahim1,2*, Ameh Danladi Amodu2, Atawodi Sunday Ene-Ojo2, Umar Alhaji Ismaila2, Mohammed Fakhruddeen3

Source: http://www.scirp.org/



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