Hypoxic Preconditioning Improved Neuroprotective Effect of Bone Marrow-Mesenchymal Stem Cells Transplantation in Acute Glaucoma ModelsReport as inadecuate




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This study explored the novel strategy of hypoxic preconditioning of Bone Marrow Mesenchymal Stem Cells BM-MSCs before intra vitreal transplantation to improve neuroprotective effects of Retinal Ganglion Cells RGCs in Acute Glaucoma Models. The methods of this research were isolated mesenchymal stem cells from the bone marrow of adult wild-type Sprague-Dawley SD rats. BM-MSCs were cultured under normoxic or hypoxic 1% oxygen for 24 hours conditions. Normoxic or hypoxic BM-MSCs were transplanted intravitreally 1 week after ocular hypertension induction by acutely increasing IOP to 100 - 120 mmHg for 60 minutes. Rats were killed 4 weeks after transplanted. Apoptosis was examined by tunnel assay and expression Brn3b Brn3b = RGCs marker by immunohistochemical analysis of the retina. Results showed that transplantation of hypoxic preconditioning BM-MSCs in acute glaucoma models resulted in a significant apoptosis decreasing p < 0.05 and an significant increasing in RGCs p < 0.05, as well as enhanced mor-phologic and functional benefits of stem cell therapy versus normoxic BM-MSCs transplantation. Conclusions: Hypoxic preconditioning enhances the capacity of BM-MSCs transplantation to improve neuroprotective effects of RGCs in Acute Glaucoma Models.

KEYWORDS

Hypoxic Preconditioning, Transplantation, Bone Marrow-Mesenchymal Stem Cells, BM-MSCs, Glaucoma, Neuroprotective

Cite this paper

Ernawati, T. , Suhendro, G. , Sudiana, I. , Putra, S. , JM, H. , Sunarjo,  . , Turchan, A. and Rantam, F. 2016 Hypoxic Preconditioning Improved Neuroprotective Effect of Bone Marrow-Mesenchymal Stem Cells Transplantation in Acute Glaucoma Models. Journal of Biomedical Science and Engineering, 9, 245-257. doi: 10.4236-jbise.2016.94018.





Author: Titiek Ernawati1,2, Gatut Suhendro3,4, I Ketut Sudiana5, Suhartono Taat Putra5, Harjanto JM6, Sunarjo7, Agus Turchan4,8, Fedik Ab

Source: http://www.scirp.org/



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