Incorporation of Ascorbic Acid, Caffeine and Chloroquine Diphosphate in Dilutor Improves Structural and Functional Status of Frozen SemenReport as inadecuate




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Effect of supplementation of ascorbic acid, caffeine and chloroquine diphosphate in the dilutor on post-thaw quality of spermatozoa was studied. Thirty-two semen ejaculates 8 each from 2 Holstein Friesian and 2 crossbred; 50% Friesian x 50% Haryana were studied. Ascorbic acid, caffeine and chloroquine diphosphate were added in Tris-egg yolk-glycerol control extender at the concentration of 10 mM, 0.54 mM and 7 mM, respectively. The post-thaw semen parameters studied were structural acrosomal integrity and livability, functional motility, per cent sperm responding to hypoosmotic swelling test, i.e. HOS positive sperm and penetrability of sperm in bovine cervical mucus, i.e. sperm penetration distance-SPD and measurement of activities of Glutamic oxaloacetic transaminase GOT, Glutamic pyruvic transaminase GPT, Alkaline transaminase AKP and Acid transaminase ACP. Certain enzymes in the seminal plasma. The semen from crossbred bull had significantly lower mass activity P ≤ 0.01, initial progressive motility P ≤ 0.01, sperm penetration distance SPD value P ≤ 0.01, HOS positive sperm and seminal plasma GOT activity, but higher abnormal sperm. Incorporation of additives in the dilutor significantly improved the post-thaw semen quality, especially the post-thaw motility, post-thaw per cent live sperm, sperm penetration distance and sperm responding to hypo-osmotic solution. The leakage of all the 4 enzymes GOT, GPT, ACP and AKP was significantly less in the samples frozen in presence of ascorbic acid and chloroquine as compared to control. Ascorbic acid resulted in maximum improvement to the quality of frozen-thawed semen followed by chloroquine and caffeine. The study concludes that post-thaw quality of frozen semen of HF and crossbred bulls significantly improves if the dilutor is supplemented with 10 mM concentration of ascorbic acid.

KEYWORDS

Semen; Freezing; Ascorbic Acid; Caffeine; Chloroquine; Sperm Penetration Distance; Hypo-Osmotic Swelling

Cite this paper

Srivastava, S. and Kumar, S. 2014 Incorporation of Ascorbic Acid, Caffeine and Chloroquine Diphosphate in Dilutor Improves Structural and Functional Status of Frozen Semen. Open Access Library Journal, 1, 1-12. doi: 10.4236-oalib.1100011.





Author: S. Srivastava1, Satish Kumar2

Source: http://www.scirp.org/



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