ISSR Fingerprinting to Ascertain the Genetic Relationship of Curcuma sp. of TripuraReport as inadecuate




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Molecular fingerprints of four different species of Curcuma, viz., C. amada, C. caesia, C. longa and C. zedoaria, found in Tripura were developed using Inter Simple Sequence Repeats. Twenty ISSR primers generated 116 loci amplified in the range of 200 - 5000 bp with an average of 5.8 alleles and 1.6 effective alleles per locus. The percentage of polymorphic band was found to be 86.29 with an average of 5.15 per primer. Based on UPGMA algorithm these four species are placed in two different clusters that validate the classification based on external and internal morphological characters. The polymorphic ISSR markers generated from this study will be useful for understanding the genetic relationship of different species of the genus Curcuma.

KEYWORDS

Curcuma, Genetic Diversity, Inter Simple Sequence Repeats, Polymerase Chain Reaction, Taxonomy, Zingiberaceae

Cite this paper

Saha, K. , Sinha, R. , Basak, S. and Sinha, S. 2016 ISSR Fingerprinting to Ascertain the Genetic Relationship of Curcuma sp. of Tripura. American Journal of Plant Sciences, 7, 259-266. doi: 10.4236-ajps.2016.72025.





Author: Kishan Saha1, Rabindra Kumar Sinha1, Surajit Basak2, Sangram Sinha1*

Source: http://www.scirp.org/



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