Secondary Structure of a Conserved Domain in the Intron of Influenza A NS1 mRNAReport as inadecuate




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Influenza A virus is a segmented single-stranded −RNA virus that causes substantial annual morbidity and mortality. The transcriptome of influenza A is predicted to have extensive RNA secondary structure. The smallest genome segment, segment 8, encodes two proteins, NS1 and NEP, via alternative splicing. A conserved RNA domain in the intron of segment 8 may be important for regulating production of NS1. Two different multi-branch loop structures have been proposed for this region. A combination of in vitro chemical mapping and isoenergetic microarray techniques demonstrate that the consensus sequence for this region folds into a hairpin conformation. These results provide an alternative folding for this region and a foundation for designing experiments to probe its functional role in the influenza life cycle.



Author: Salvatore F. Priore, Elzbieta Kierzek, Ryszard Kierzek, Jayson R. Baman, Walter N. Moss, Lumbini I. Dela-Moss, Douglas H. Turner

Source: http://plos.srce.hr/



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