Isoenzyme Expression in Bean Seed Germination Treated with Thiamethoxam with and without Drought StressReport as inadecuate




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The thiamethoxam acts as enhancer, allowing maximizing the expression of seed vigor. Isozymesare products of gene expression, highly influenced by the environment, because genes that controlits expression are manifested in certain stages of development and in specific organs and tissues,or even under the action of certain stimulus. This study evaluated the isoenzyme expression inbean seedlings from seeds treated with thiamethoxam. Bean seeds were used, cultivar Pérola andIAPAR Siriri, submitted or not to drought stress. Seeds were treated at doses of thiamethoxam: 0, 1,2, 3, 4 and 5 mL·kg-1 of seed. Isozymes were extracted from seedlings collected five days aftersowing. We evaluated the expression of isoenzymes: Glutamate Oxalacetate transaminase GOT,acid phosphatase FAC, peroxidase PO, Esterase EST, malate dehydrogenase MDH and glutamatedehydrogenase GTDH. Interpretation of the results was based on visual analysis of electrophoresisgels, considering the presence-absence and the intensity of each electrophoretic band.Bean seeds treated with thiamethoxam with doses up 3 mL·kg-1 of seed are higher germinationuntreated, even when submitted to drought stress. The expression of isozymes GOT, FAC, PO, EST,MDH and GTDH is not affected significantly in bean seedlings of Pérola and IPR Siriri under differentdoses of thiamethoxam with and without drought stress.

KEYWORDS

Phaseolus vulgaris, Enzymes, Germination, Bioactivator

Cite this paper

Villela, A. , Almeida, A. , Castellanos, C. , Deuner, C. , Soares, V. , Camargo, T. , Meneghello, G. , Villela, F. , Tunes, L. and Zimmer, P. 2015 Isoenzyme Expression in Bean Seed Germination Treated with Thiamethoxam with and without Drought Stress. American Journal of Plant Sciences, 6, 3157-3163. doi: 10.4236-ajps.2015.619307.





Author: Arthur Blois Villela1, Andreia da Silva Almeida2, César Iván Suárez Castellanos2, Cristiane Deuner2, Vanessa Nogueira Soares2,

Source: http://www.scirp.org/



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