C-Jun N-Terminal Kinase JNK Mediates Wnt5a-Induced Cell Motility Dependent or Independent of RhoA Pathway in Human Dental Papilla CellsReport as inadecuate




C-Jun N-Terminal Kinase JNK Mediates Wnt5a-Induced Cell Motility Dependent or Independent of RhoA Pathway in Human Dental Papilla Cells - Download this document for free, or read online. Document in PDF available to download.

Wnt5a plays an essential role in tissue development by regulating cell migration, though the molecular mechanisms are still not fully understood. Our study investigated the pathways involved in Wnt5a-dependent cell motility during the formation of dentin and pulp. Over-expression of Wnt5a promoted cell adhesion and formation of focal adhesion complexes FACs in human dental papilla cells hDPCs, while inhibiting cell migration. Instead of activating the canonical Wnt signal pathway in hDPCs, Wnt5a stimulation induced activation of the JNK signal in a RhoA-dependent or independent manner. Inhibiting JNK abrogated Wnt5a-induced FACs formation but not cytoskeletal rearrangement. Both dominant negative RhoA RhoA T19N and constitutively active RhoA mutants RhoA Q63L blocked the Wnt5a-dependent changes in hDPCs adhesion, migration and cytoskeletal rearrangement here too, with the exception of the formation of FACs. Taken together, our study suggested that RhoA and JNK signaling have roles in mediating Wnt5a-dependent adhesion and migration in hDPCs, and the Wnt5a-JNK pathway acts both dependently and independently of the RhoA pathway.



Author: Chenglin Wang, Yuan Zhao, Yingying Su, Ruimin Li, Yunfeng Lin, Xuedong Zhou, Ling Ye

Source: http://plos.srce.hr/



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