Unlipidated Outer Membrane Protein Omp16 U-Omp16 from Brucella spp. as Nasal Adjuvant Induces a Th1 Immune Response and Modulates the Th2 Allergic Response to Cow’s Milk ProteinsReport as inadecuate




Unlipidated Outer Membrane Protein Omp16 U-Omp16 from Brucella spp. as Nasal Adjuvant Induces a Th1 Immune Response and Modulates the Th2 Allergic Response to Cow’s Milk Proteins - Download this document for free, or read online. Document in PDF available to download.

The discovery of novel mucosal adjuvants will help to develop new formulations to control infectious and allergic diseases. In this work we demonstrate that U-Omp16 from Brucella spp. delivered by the nasal route i.n. induced an inflammatory immune response in bronchoalveolar lavage BAL and lung tissues. Nasal co-administration of U-Omp16 with the model antigen Ag ovalbumin OVA increased the amount of Ag in lung tissues and induced OVA-specific systemic IgG and T helper Th 1 immune responses. The usefulness of U-Omp16 was also assessed in a mouse model of food allergy. U-Omp16 i.n. administration during sensitization ameliorated the hypersensitivity responses of sensitized mice upon oral exposure to Cow’s Milk Protein CMP, decreased clinical signs, reduced anti-CMP IgE serum antibodies and modulated the Th2 response in favor of Th1 immunity. Thus, U-Omp16 could be used as a broad Th1 mucosal adjuvant for different Ag formulations.



Author: Andrés E. Ibañez, Paola Smaldini, Lorena M. Coria, María V. Delpino, Lucila G. G. Pacífico, Sergio C. Oliveira, Gabriela S. R

Source: http://plos.srce.hr/



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