Alternative Splicing of the Amelogenin Gene in a Caudate Amphibian, Plethodon cinereusReport as inadecuate




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As the major enamel matrix protein contributing to tooth development, amelogenin has been demonstrated to play a crucial role in tooth enamel formation. Previous studies have revealed amelogenin alternative splicing as a mechanism for amelogenin heterogeneous expression in mammals. While amelogenin and its splicing forms in mammalian vertebrates have been characterized, splicing variants of amelogenin gene still remains largely unknown in non-mammalian species. Here, using PCR and sequence analysis we discovered two novel amelogenin transcript variants in tooth organ extracts from a caudate amphibian, the salamander Plethodon cinereus. The one was shorter -S- 416 nucleotides including untranslated regions, 5 exons and the other larger -L- 851 nt, 7 exons than the previously published -normal- gene in this species -M- 812 nucleotides, 6 exons. This is the first report demonstrating the amelogenin alternative splicing in amphibian, revealing a unique exon 2b and two novel amelogenin gene transcripts in Plethodon cinereus.



Author: Xinping Wang , Zeli Xing, Xichen Zhang, Lisai Zhu, Thomas G. H. Diekwisch

Source: http://plos.srce.hr/



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