Temporal and Spatial Changes in the Pattern of Iba1 and CD68 Staining in the Rat Brain Following Severe Traumatic Brain InjuryReport as inadecuate




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We have previouslydemonstrated that acute treatment with low dose methamphetamine isneuroprotectivein a rat model of severe traumatic brain injury TBI. Usinggene expression analysis, we further showed that methamphetamine treatmentsignificantly reduced the expression of pro-inflammatory genes after severeTBI. Therefore, to further investigate the potential effects of methamphetaminetreatment on the neuroinflammatory response, we examined immunofluorescentstaining of Iba1 and CD68, two marker of neuroinflammation, in the rat lateralfluid percussion injury model of severe TBI. In this study, we observedtemporal and spatial alterations in the pattern of Iba1 and CD68 labelingwithin two weeks after severe TBI. In general, methamphetamine treatment didnot dramatically alter the pattern of Iba1 and CD68 staining. However, we didobserve a unique and significant drug-induced increase of Iba1 labeling withinthe granule cell layer of the dentate gyrusat 48 hours post injury. We alsoobserved rod-shaped Iba1+ cellswithin the core lesion in the cortex. These cells showed variable staining withCD68 and aligned most closely with MAP2+ neuronal processes. Thus, acutetreatment with low-dose methamphetamine after severe TBI caused a transientbilateral increase of Iba1+ cellswithin the granule layer of the dentate gyrus but did not alter the overalltemporal and regional pattern of Iba1 and CD68 staining within the cortex,periventricular white matter, fimbria, or thalamus.

KEYWORDS

TBI, Methamphetamine, Iba1, CD68

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Smith, D. , Brooks, D. , Wohlgehagen, E. , Rau, T. and Poulsen, D. 2015 Temporal and Spatial Changes in the Pattern of Iba1 and CD68 Staining in the Rat Brain Following Severe Traumatic Brain Injury. Modern Research in Inflammation, 4, 9-23. doi: 10.4236-mri.2015.42002.





Author: Debbie Smith1, Diane Brooks1, Eric Wohlgehagen1, Thomas Rau1, David Poulsen2*

Source: http://www.scirp.org/



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