Effects of Ox-LDL on Macrophages NADPH Autofluorescence Changes by Two-photon Microscopy - Physics > Biological PhysicsReport as inadecuate




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Abstract: Ox-LDL uptakes by macrophage play a critical role in the happening ofatherosclerosis. Because of its low damage on observed cells and bettersignal-to- background ratio, two-photon excitation fluorescence microscopy isused to observe NADPH autofluorescence of macrophage under differencecultured conditions- bare cover glass, coated with fibronectin orpoly-D-lysine. The results show that the optimal condition is fibronectincoated surface, on which, macrophages profile can be clearly identified onNADPH autofluorescence images collected by two-photon microscopy. Moreover,different morphology and intensities of autofluorescence under differentconditions were observed as well. In the future, effects of ox-LDL onmacrophages will be investigated by purposed system to research etiology ofatherosclerosis.



Author: Ching-Ting Lin, En-Kuang Tien, Szu-Yuan Lee, Long-Sheng Lu, Chau-Chung Wu, Chen-Yuan Dong, Chii-Wann Lin

Source: https://arxiv.org/



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