Tomato Plants Overexpressing a Celery Mannitol Dehydrogenase MTD Have Decreased Susceptibility to Botrytis cinereaReport as inadecuate




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The oxidative burst is a critical early event in plant-pathogen interactions that leads to a localized, programmed cell death PCD called the hypersensitive response HR. The HR and associated PCD retard infection by biotrophic pathogens, but can, in fact, enhance infection by necrotrophic pathogens like Botrytis cinerea. In addition to signaling the induction of the HR, reactive oxygen species ROS produced during the oxidative burst are antimicrobial. We hypothesize that pathogens such as B. cinerea survive the antimicrobial effects of ROS, at least partially by secreting the antioxidant mannitol during infection. This is supported by the previous observation that overexpression of the catabolic enzyme mannitol dehydrogenase MTD can decrease a plants susceptibility to mannitol-secreting pathogens like B. cinerea. To extend the above hypothesis, and test the general utility of this approach in an important horticultural crop, we overexpressed celery MTD in tomato Solanum lycopersicum cv. -Moneymaker-. In these studies, we observed a significant increase up to 90% in resistance to B. cinerea in transgenic tomatoes expressing high amounts of MTD.

KEYWORDS

Hypersensitive Response, Pathogenesis Response, Plant-Pathogen Interaction, Programmed Cell Death, Reactive Oxygen Species

Cite this paper

Patel, T. , Krasnyanski, S. , Allen, G. , Louws, F. , Panthee, D. and Williamson, J. 2015 Tomato Plants Overexpressing a Celery Mannitol Dehydrogenase MTD Have Decreased Susceptibility to Botrytis cinerea. American Journal of Plant Sciences, 6, 1116-1125. doi: 10.4236-ajps.2015.68116.





Author: Takshay K. Patel1, Sergei F. Krasnyanski1, George C. Allen1, Frank J. Louws2, Dilip R. Panthee1, John D. Williamson1*

Source: http://www.scirp.org/



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