RimJ-Catalyzed Sequence-Specific Protein N-Terminal Acetylation in Escherichia coliReport as inadecuate




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In order to establish the sequence dependence of RimJ-mediated protein N-terminal acetylation in E. coli, the Z-domain variants differing by the second or third amino acid residue were expressed and analyzed by mass spectrometry. Only subsequent to the initiating methionine residue cleavage, the RimJ-catalyzed N-terminal acetylation mainly occurred at the N-terminal serine and threonine residues and was significantly enhanced by hydrophobic or negatively charged residues in the penultimate position.

KEYWORDS

Protein N-Terminal Processing, Protein N-Terminal Acetylation, RimJ

Cite this paper

Perez, L. and Ryu, Y. 2015 RimJ-Catalyzed Sequence-Specific Protein N-Terminal Acetylation in Escherichia coli. Advances in Bioscience and Biotechnology, 6, 182-193. doi: 10.4236-abb.2015.63018.





Author: Lina F. Bernal- Perez, Youngha Ryu*

Source: http://www.scirp.org/



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