Heritable and Precise Zebrafish Genome Editing Using a CRISPR-Cas SystemReport as inadecuate




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We have previously reported a simple and customizable CRISPR clustered regularly interspaced short palindromic repeats RNA-guided Cas9 nuclease RGN system that can be used to efficiently and robustly introduce somatic indel mutations in endogenous zebrafish genes. Here we demonstrate that RGN-induced mutations are heritable, with efficiencies of germline transmission reaching as high as 100%. In addition, we extend the power of the RGN system by showing that these nucleases can be used with single-stranded oligodeoxynucleotides ssODNs to create precise intended sequence modifications, including single nucleotide substitutions. Finally, we describe and validate simple strategies that improve the targeting range of RGNs from 1 in every 128 basepairs bps of random DNA sequence to 1 in every 8 bps. Together, these advances expand the utility of the CRISPR-Cas system in the zebrafish beyond somatic indel formation to heritable and precise genome modifications.



Author: Woong Y. Hwang , Yanfang Fu , Deepak Reyon, Morgan L. Maeder, Prakriti Kaini, Jeffry D. Sander, J. Keith Joung, Randall T. Peters

Source: http://plos.srce.hr/



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