Dynamic Light Scattering from Oriented, Rotating Particles: A Theoretical Study and Comparison to Electrorotation DataReport as inadecuate




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Abstract : In recent years, electrorotation has developed in the field of biology as a technique for characterization of single cell dielectric properties. Applications to colloidal particles are scarce, although the method provides information on the electrical structure of the particle-s interior. The method explores the frequency-dependent polarizability difference of the particles and the suspension medium by using rotating field in the frequency range of 1 kHz to 200 MHz to induce individual particle rotation. To allow interpretation of the electrorotation spectra measured with light scattering techniques, the theoretical autocorrelation function of light scattered from rotating particles of cylindrical symmetry has been calculated. All particles were assumed to possess a single scattering site and the rotation axes were assumed to be parallel. An appropriate expression for the rotational diffusion around the longitudinal angle was also derived. Diffusion around the azimuthal angle could be neglected. The theoretical result consists of a well structured autocorrelation function. The theoretical limits of particle size and rotation speed that allow detection of electrorotation were explored. Experimental autocorrelation functions from electrorotation of human red blood cells could be explained theoretically when reasonable parameters for the cell-s properties were assumed.





Author: Peter Eppmann Jan Gimsa Bernhard Prüger Edwin Donath

Source: https://hal.archives-ouvertes.fr/



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