The Effect of Electroacupuncture on Neuronal Apoptosis and Related Functions in Rats with Acute Spinal Cord InjuryReport as inadecuate




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Objective: To investigate the effect and significance of electroacupuncture EA on neuronal apoptosis and hindlimb motor and bladder functional improvement in rats with acute spinal cord injury SCI. Methods: Sixty healthy Sprague Dawley rats were randomly assigned to sham, model, EA, and EA control groups n = 15 each. EA group rats received EA treatment at Zhibian and Shuidao acupoints seven times daily, whereas EA control group rats received EA at two points, 0.5 cm away from Zhibian and Shuidao, respectively. Histomorphological changes in spinal cord tissue were examined using hematoxylin-eosin staining. Neuronal apoptosis was detected by TUNEL assay. Bcl-2, Bax, and Bad protein levels were detected using immunohistochemistry. Additionally, hindlimb motor function, residual urine volume and maximum bladder capacity were measured. Results: HE staining revealed a morphologically and structurally intact spinal cord in the EA group, and the tissue contained scattered blood cells without edema. In the EA control group, there were small morphological defects in the spinal cord, and the tissue contained fewer blood cells with local edema. Compared with the EA control and model groups, Bax and Bad levels were significantly decreased in the EA group and Bcl-2 expression was increased P < 0.05. After SCI, hindlimb function scores, residual urine volume, and maximum bladder capacity in rats of the EA group significantly differed from those of the EA control group P < 0.05. Conclusion: EA may induce SCI-induced improvements in hindlimb motor and bladder functions by affecting neuronal apoptosis and relevant gene expression changes.

KEYWORDS

Electroacupuncture, Spinal Cord Injury, Neuronal Apoptosis, Molecular Mechanism

Cite this paper

Zhang, L. , Li, C. , Quan, R. and Xie, S. 2014 The Effect of Electroacupuncture on Neuronal Apoptosis and Related Functions in Rats with Acute Spinal Cord Injury. Chinese Medicine, 5, 199-210. doi: 10.4236-cm.2014.54024.





Author: Liang Zhang1, Changming Li1, Renfu Quan2*, Shangju Xie1

Source: http://www.scirp.org/



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