Evaluation of Acute and Repeated Dose Toxicity of the Polyherbal Formulation Linkus Syrup in Experimental AnimalsReport as inadecuate




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The objectiveof the present study was to evaluate the pre-clinical efficacy and toxicity of polyherbalcough syrup Linkus. Method: Animals healthy Wistar albino rats; 150 - 250 g ofeither sex were housed under standard environmental conditions; i.e. 25°C ± 1°C and 12 h dark-light cycle.Food and water were available at libitum.The rats were treated orally with the recommended doses of the test drug Linkus.After 15 minutes, they were individually placed in a closed Plexiglas chamber 20× 10 × 10 cm and exposed to citric acid 0.1 g-ml inhalation for 7 minutes. Thecough reflexes were produced and counted for the last 5 minutes and compared withthose of the control animals. The following studies were conducted to evaluate thetoxicity of the test drug in healthy Wistar albino rats: lethal dose50 LD50;rats of either sex n = 10-sex were treated orally with doses 1 or 5 g-kg ofthe test drug. Mortality and behavioral changes were observed for 1 week. Repeateddose toxicity on the healthy Wistar albino rats of both sexes n = 5-dose-sex wastreated orally with doses of 20 mg-kg adult human dose = ~1400 mg, 500 mg-kg adulthuman dose = ~35,000 mg and 1000 mg-kg adult human dose = ~70,000 mg of testdrug Linkus for 14 days. Additionally, the control animals were treated orallywith water for 14 days. Results: In female rats, the test drug Linkus at the doseof 300 mg-kg caused significant p < 0.01 reduction in the cough reflexes ascompared to the control. However, in male rats, a significant reduction was observedat the tested dose of 200 mg-kg p < 0.05 and 300 mg-kg p < 0.01. The testproduct did not cause mortality in rats at the given doses of 1 or 5 g-kg. Othersigns of toxicity like hair loss and weight reduction were not observed. In femaleand male rats, the test drug Linkus at different doses did not show any abnormaleffects on complete blood count profile of rats. Serum enzyme markers, i.e. alanine aminotransferase ALT, alakalinephosphatase, gamma glutamyle transferase GGT, direct bilirubin, creatinine, andproteins were also observed and found that the test drug at a higher dose did notcause any of the abnormality and had shown significant p value as compared to thecontrol. Conclusion: The test drug Linkus could be an effective and safe coughsyrup because it did not show any of the side effects or toxicity on experimentalanimals.

KEYWORDS

Cough Expectorant, Alanine Amino Transferase ALT, Polyherbal, Gamma Glutamyl Transferase GGT, Toxicity

Cite this paper

Nawaz, A. , Bano, S. , Sheikh, Z. , Usmanghani, K. , Ahmad, I. , Zaidi, S. , Zahoor, A. and Ahmad, I. 2014 Evaluation of Acute and Repeated Dose Toxicity of the Polyherbal Formulation Linkus Syrup in Experimental Animals. Chinese Medicine, 5, 179-189. doi: 10.4236-cm.2014.54022.





Author: Allah Nawaz1,2, Saira Bano1, Zeeshan Ahmed Sheikh1, Khan Usmanghani1,3*, Iqbal Ahmad4, Syed Faisal Zaidi5, Aqib Zahoor1, Irshad A

Source: http://www.scirp.org/



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